Coding
T7 Immedia

Part:BBa_K1477030:Design

Designed by: 2014 Ivy Tech South Bend IN team   Group: iGEM14_IvyTech_SouthBend_IN   (2014-08-22)


T7 strong promoter + endolysin cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 345
    Illegal AgeI site found at 415
  • 1000
    COMPATIBLE WITH RFC[1000]


=Design Notes

This part was built to short circuit bacteriophage infection in a host cell; Infection by phage was designed to result in immediate lysis with no procession of the infectious cycle.

A typical phage replication cycle is represented here: Phage life cycle.png

BBa_K1477030 was designed to short circuit coliphage T7 infection Phage Immediate Lysis.png

Source

T7 virus and Endolysin

References